0 1 . 1 Describe the induced-fit model of enzyme action and how an enzyme acts as a catalyst. Scientists investigated the action of the enzyme ATP synthase. They made reaction mixtures each conta... ining: •ATP synthase •buffer (to control pH) •substrates. One of the substrates required in these reaction mixtures is inorganic phosphate (Pi). Tick () one box to show which other substrate the scientists must add to the reaction mixtures to produce ATP. [1 mark] Adenine Adenosine diphosphate Glucose Ribose The scientists investigated the effect of concentration of inorganic phosphate (Pi) on ATP synthase activity. After 2 minutes, they stopped each reaction and then measured the concentration of ATP. Figure 1 shows the scientists’ results. Crystallin is a structural protein found in the human eye. An inherited disease that leads to blindness is caused by changes in properties of crystallin. The replacement of the amino acid Arg with the amino acid Gly causes these changes. Use information in Table 2 to suggest why this amino acid replacement changes the properties of crystallin. [Show More]
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