Portage Learning BIO 171- Microbiology Lab Notebook

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Portage Learning BIO 171- Microbiology Lab Notebook Lab Notebook Bookmarks (click to navigate): Lab 1 Notebook Lab 2 Notebook Lab 3 Notebook Lab 4 Notebook Lab 5 Notebook Lab 6 Notebook Lab ... 7 Notebook Lab 8 Notebook Lab 9 Notebook Lab 1 Notebook Back to Home Page Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation of samples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation of samples: microscopy- visualization and key characteristics of specimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure it is depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotates to aerateliquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria Outline of how to set up Notebook Objective: To establish an organized template for keeping experimental records, procedures, and results. Procedure: 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment- put them in red Notes: Additional (helpful) information placed here Results: A summary of the final outcome of the experiment should be described here Lab 2 Notebook Back to Home Page Title: KD02 Introduction to Microscopy Objective: To learn basic parts of microscope and how to view a sample Procedure: 1. Review the parts of microscope 2. Load sample to be viewed 3. Choose magnification 4. Adjust microscope so the sample is clear Notes: Parts of microscope: 1. eye pieces (ocular lens): can be pulled apart; should be able to use both eyes and see one circle if not adjust 2. arm/neck: if moving this is what you will grab with one hand and hand under base 3. Objective lenses: this provides the magnification of the sample; shortest has the least magnification (4x, 10x, 40x,100x) 4. Stage: the flat surface that you place your sample; holds the sample via a clamp (squeeze together to place sample and release); stage clips raise up and lay on the glass coverslip 5. Focus knobs: located on the side of the arm/neck; Outside ring is the coarse adjustment – makes large ste [Show More]

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