BIO 171- Microbiology Lab Notebook-All labs-1 to 9-comprehensive-2022-2023

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Lab Notebook Bookmarks (click to navigate): Lab 1 Notebook Lab 2 Notebook Lab 3 Notebook Lab 4 Notebook Lab 5 Notebook Lab 6 Notebook Lab 7 Notebook Lab 8 Notebook Lab 9 Notebook... Lab 1 Notebook Back to Home Page Title: KD01 Introduction to medical microbiology & lab notebook procedures Course Objectives: Cultivation of samples: growth conditions and equipment used Identification of samples: biochemical assays & tests available Evaluation of samples: microscopy- visualization and key characteristics of specimens Basic Equipment: Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take minutes to sterilize & hot dry air which will take hours; before opening make sure it is depressurized and will have very hot air. Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes - Shaker incubator 37°C shakes culture & rotates to aerate- liquid nutrient broth Visualizing: microscopy Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term Lab Safety 1. Never eat or drink in the lab: contamination risks 2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal, or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills 3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria Outline of how to set up Notebook Objective: To establish an organized template for keeping experimental records, procedures, and results. Procedure: 1. This is where each step of a given protocol is recorded 2. Be sure to clearly indicate any deviations that may occur during the experiment- put them in red Notes: Additional (helpful) information placed here Results: A summary of the final outcome of the experiment should be described here CONTINUED...... [Show More]

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