Microbiology > Lab Experiment > MICROBIOLOGY LAB 1 ,9 (All)
TK01 Keeping a Lab Notebook Microbiology Lab1 Course Objectives: Cultivation of samples (growth conditions) - equipment used Identification of samples (biochemical assays) - tests available Evaluat... ion of samples (microscopy) - visualizations and recognitions key concepts Basic Equipment: 1. Cleaning: autoclave 125 ° C, steamed, heat and pressure sterilization, chamber with thick door, not dry air but steamed - more efficient, make sure depressurized before opening Time restrictions: no autoclave then use steam (minutes) vs. hot air (hours) 2. Growing: fixed incubator = 37 ° C; shaker incubator = 37 ° C, for growth mediator, rotates and shakes in circular motions to aerate and help optimize bacterial growth 3. Visualizing: Microscopy, can't see with just the naked eye, must know parts of microscope 4. Storing: Refrigerate at 4 ° C, slows bacterial growth, prolongs life of sample at this temperature, when not finished with experimentation Lab Safety: 1. Never eat or drink in lab - contamination risks 2. Wear Personal Protective Equipment (PPE): Use gloves: latex, nitrile (hypoallergenic) purple,blue or green, cold thermal - liquid nitrogen, etc., heat thermal resistant gloves - extreme hot conditions Eyewear protection - goggles Lab coat - dependent on working with materials that may be spilt on clothes 3. NEVER leave lab while wearing PPE includes: Public Hallway, Restroom, Cafeteria Lab Notebook: First Name Initial, Last name initial, Experiment #, Title of Experiment Example: TK01 Keeping a Lab Notebook Objective: Developing an organized lab notebook for documenting experiments, data, procedures and records. [Show More]
Last updated: 6 months ago
Preview 1 out of 41 pages
Connected school, study & course
About the document
Uploaded On
Nov 06, 2023
Number of pages
41
Written in
This document has been written for:
Uploaded
Nov 06, 2023
Downloads
0
Views
43
In Browsegrades, a student can earn by offering help to other student. Students can help other students with materials by upploading their notes and earn money.
We're available through e-mail, Twitter, Facebook, and live chat.
FAQ
Questions? Leave a message!
Copyright © Browsegrades · High quality services·