Micro Biology > Exam > STRAIGHTERLINE Microbiology BIO 250L Lab 7 Microbial Control 2022 COMPLETE LAB WORKSHEET (All)

STRAIGHTERLINE Microbiology BIO 250L Lab 7 Microbial Control 2022 COMPLETE LAB WORKSHEET

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1. Which germicide was the most effective? Was the same germicide most effective for bacteria isolated from the different sources? What could explain this finding? 10% bleach was most effective. Yes... , it was most effective for bacteria isolated from the different sources. Since bleach is a solution of sodium hypochlorite and water that is very effective in killing microorganisms, it is capable of entering microbial cells where it can react with many cellular components, disrupting and killing them. 2. Which germicide was the least effective? Was the same germicide least effective for bacteria isolated from different sources? What could explain this finding? 70% Isopropyl alcohol was least effective. Yes, it was least effective on the bacteria isolated frlm different places. This could be because, it erodes quicklg if left uncapped, evaporating most of its alcohol leaving behind only water which is not strong enough to destroy bacteria.Lab 7 Microbial Control BIO250L 3. Were any bacteria from a specific source most resistant to the different germicides? If so, which source? The germicidal resistance possessed of the gram-negative bacteria and gram-positive bacteria was the same, except for aeruginosa which was more resistant to most disinfectants in its naturally occuring state than most cells subcultured on labaratory media. Also pseudomonas aeruginosa too exhibited greater resistance to some disinfectants. 4. Did the dry filter paper serve as a positive or negative control? Negative control. 5. Look up the chemical formulas for bleach, isopropyl alcohol, and chlorhexidine gluconate (the active ingredient in Hibiclens). How does each chemical achieve its germicidal properties? [Show More]

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